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KMID : 0382420030290030072
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Korean Journal of Environmental Health Society
2003 Volume.29 No. 3 p.72 ~ p.78
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Optimization of Substract Concentration in Cell Production of Fungal Chitosan
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Kim Bong-Seob
Suh Myung-Gyo
Roh Jong-Su
Lee Yong-Hee
Lee Kook-Eui
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Abstract
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In the process of producing chitosan from crustacean shell. the use of excessive acid and alkli is causing the problems of environmental pollution and of production cost. In this study. one way to solve these problems is to cultivate fungi. then. to extract chitosan from the cell wall. By means of flask incubation and batch cultivation. the optimum cultivation conditions for mass production of continuous cultivation was found. Four strains used for the production of fungal chitosan were Gongtronella beri IFO8080. Absidia coe겨lea IFO5301. Rhizopus delemar IFO4775. Mucor tuberculispo겨s IFO9256. In flask incubation to select strain of producing much chitosan by means of experiment of the effect of initial pH. Absidia coelea IFO 5301 had highest yield in FCs. 258.1±47.3 ㎎/200 m/ at pH 6.5. In flask incubation under the optimum cultivation condition. temperature 27℃, culture time 6days. glucose 2%. peptone 1%. (NH₄)₂SO₄0.5%, K₂HPO₄ 0.1%. Nacl 0.1 %. MgSO₄7H₂O 0.05%. CaCI₂ 2H₂O, 0.01%. the yield of DCW brought the highest yields. In batch bioreactor. the optimum cultivation condition was that cell suspended solution was 70 ml. aeration rate 0.5 l/min. agitation rate 800rpm. culture time 36hr. In continuous bioreactor. the optimum substrate flow rate was 4l/day.
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